PCR-RLFP on IGFBP -3 gene and its association on growth performance of lambs reared intensively

IGFBP-3 is responsible for the multiple effects of growth factors in most mammalian species and considered major transport factor growth, used as a marker different body functions such metabolism, reproduction, weight control, immunity, energy balance, so on. Considered candidate gene, production traits its essential role development animals. For DNA extraction, blood samples are obtained jugular vein using 10 ml vacutainer containing EDTA coagulant collection each animal Mis breed sheep (M), Ile de France (F), Wurttemberg (W). Isolation performed extraction kit (Quick kit) with primers set Forward Reverse. The weights lambs from birth to 90 days age, also been calculated. results agarose gel electrophoresis PCR amplified genes populations (W), (F) had 654 bp. In our showed an absence polymorphism gene on tested populations. that there no between examined breeds, terms genes, we were interested whether differences mentioned because related All three breeds have similar dynamics, which could link hormone. Analyzing results, can suggest large difference W, M, F does not be but other genetic non-genetic affect this trait. To detect association lambs, sequencing required, will subject future research.